What it is

Virus Detection and Prevention

The financial impact of an undetected pathogen like Hop Latent Viroid (HLVd) can be devastating for a cannabis grower. Symptoms of HLVd can be subtle and difficult to perceive. One infected Mother plant could contaminate hundreds of adjacent plants or clones resulting in slower growth, lower potency, and stunted yield by up to 70% with a loss of potency and terpene production up to 35%.

As with most obligate pathogens, prevention is key. And prevention starts with early detection. The most reliable and sensitive means of detection is accomplished by viral RNA testing. MH Verify is the first rapid field test kit to help growers identify and mitigate the spread of pathogens like HLVd in cannabis and hemp plants.

MH Verify’s proven field test kit makes it easier for growers to detect plant pathogen contamination rapidly and privately.

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Benefits

Why Buy MH Verify

Revenue

If a single cannabis plant produces 3 pounds of flower at an average price of $2,000 per pound, the potential revenue of that one plant is $6,000 per harvest to a grower. An HLVd infected plant could reduce the plant’s yield by up to 70% or $4,000 in reduced revenue. And because pathogens like HLVd are difficult to detect and easily transmissible through touch or cloning, one infected plant can contaminate hundreds if not thousands of plants. MH Verify can help a grower optimize a cannabis plant’s yield and protect hundreds if not thousands of healthy, income-producing plants.

Speed

The key to mitigating the spread of plant viruses is rapid and accurate detection. Currently growers do not have many options to test for HLVd. Growers can send samples to a laboratory but often wait weeks for these test results. The other alternative is to bring testing in house, but this requires a significant capital expense and staff training until now. MH Verify provides faster and reliable HLVd virus detection within hours not weeks. Growers can now correlate plant phenotypes with specific diseases with accuracy. Starter and Refill kits are quickly shipped directly to the cultivation site, and within two hours delivers verified results.

Privacy

Growers can ill afford for an extractor and manufacturer to hear that their cannabis plants are contaminated. By sending plants to specific testing labs, Certificates of Analysis will reveal this information. MH Verify enables growers to address this issue in-house before sending samples to labs for analysis. And if other cultivators catch wind a competitor’s contaminated plants, reputations and entire harvests will be at stake. Thanks to MH Verify, growers can proactively protect their reputation and harvest.

Ease of Use

MH Verify is as easy as a home pregnancy test. Growers can mitigate infection and cross-contamination of plant pathogens in the field and completely on their own. The Starter Kit provides all the equipment and testing tools to run 24 tests. Simple step-by-step instructions and diagrams are also included. Watch the video below to see how easy MH Verify is to use. Refill Kits can be ordered separately or with the Starter Kit, and shipped quickly to ensure your ongoing monitoring program is as timely and effective as it can be.

Cost-Effective

Some alternative thermocyclers can cost over $50,000. Microbiologists earn a high wage to run these thermocyclers and isolate RNA. These costs can be over $100,000 per year. With MH Verify, growers do not need to be a Microbiologist to conduct these detection tests. MH Verify costs $900 for the Starter Kit and $700 for a Refill Kit. Growers can now save significant capital expenses without sacrificing testing speed and reliability. MH Verify is the most cost-effective all-in-one solution on the market today.

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Products

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Starter Kit

The Starter Kit rapid test is designed for in-field detection of HLVd viruses to allows for disease detection which in turns aids faster decision making during the plant disease management process. The Starter Kit provides all the equipment and testing tools needed by growers to run 24 tests. Step-by-step instructions with intuitive diagrams are also included.

IN THIS KIT

20 ML Vial with Beads and Extractant (qty 24)
1.5 ML Tubes with Lyophilized Reaction (qty 24)
Large Transfer Pipette (qty 24)
Small Transfer Pipette (qty 24)
Dilution Buffer (qty 1)

Test Strips (qty 24)
Scissors (qty 1)
Forceps (qty 1)
Incubator (qty 1)
Rack (qty 1)

NOTE: Shipping not included, FedEx Ground or FedEx 2-Day are available. Lighter and gloves not included.

Refill Kit

The Refill Kit allows for HLVd disease detection which helps growers save time and money. This kit provides all the replacement tools necessary to perform 24 tests. Step-by-step instructions with intuitive diagrams are also provided.

IN THIS KIT

20 ML Vial with Beads and Extractant (qty 24)
1.5 ML Tubes with Lyophilized Reaction (qty 24)
Large Transfer Pipette (qty 24)
Small Transfer Pipette (qty 24)

Dilution Buffer (qty 1)
Test Strips (qty 24)

NOTE: Shipping not included, FedEx Ground or FedEx 2-Day are available. Lighter and gloves not included.

How it Works

See Results Within Two Hours

Frequently Asked Questions

What is Hop Latent Viroid (HLVd)?

Hop Latent Viroid (HLVd) is a single-stranded, circular strand of RNA with no protein coat. This Hop Latent Viroid can cause disease in many plants, including cannabis plants. The phenotype of an infected cannabis plant may be referenced as “duding,” “stunting,” or “dudding disease.” Plants infected with HLVd may or may not show symptoms (symptomatic vs asymptomatic) and the disease can be dormant in a plant for extended periods of time prior to showing symptoms.

How can you tell if the plant is infected?

HLVd noticeable symptoms in cannabis plants include:

  • Growth stunting
  • Abnormal branching and brittle stems
  • Reduced trichomes, potency and oil production
  • Reduced flower mass
  • Malformation and/or Chlorosis of the leaves

How does this impact me as a grower?

HLVd will greatly reduce the quality and quantity of the flower the infected plant produces. During the vegetative stage, plants will grow shorter with smaller leaves and tighter node spacing. Flowering plants will have smaller looser buds with much fewer trichomes. Over time, yields can be reduced up to 70% with a loss of potency and terpene production up to 35%.

How do the plants get infected?

HLVd infects plants mostly through cross contamination. It is typically spread via:

  • Infected pruning tools, which is why cultivators should always sterilize their equipment before starting work on a new plant
  • Cloning when cuttings are taken from an infected mother
  • Contaminated seeds
  • Contaminated clothing and physical touching.

How do you test for the viroid?

MH Verify offers both a “Starter” Test Kit and the “Refill” Test Kit. Both kits include instructions and all the equipment and tools necessary to perform tests. To view a video of the Starter Kit, visit [insert link].

How long does it take to do the test and get results?

Approximately 2 hours from unpacking kit to results.

Can someone assist me with the testing in the field?

Our HLVd testing process is as simple as a Home Pregnancy Test and should not require any on-site assistance. But all customers should reach out to their MH Verify representative whenever they think guidance in warranted.

Can your lab do the testing for us?

We are currently developing an in-house testing procedure. (No pricing available yet)

What if my plants tested positive, what do I do then?

Because HLVd is a relatively new cannabis issue, there isn’t a current universally accepted standard treatment, but growers are experimenting with various treatments. The most common experimental treatments that we are currently aware of include:

  • Plant Cleaning: Preparing a broad-spectrum bactericide/fungicide (e.g. Zerotol) and dunking a freshly cut clone and after rooting, re-dunk the clone in a broad-spectrum bactericide/fungicide.
  • Plant Spraying with Regalia and/or Axiom

Am I required to document any HLVd issues to state regulators or any other governing authorities?

No. This test is purely so that you can address internal issues at your cultivation site and maximize your profitability.

How do I test using your kit?

The kit’s instructions read as follows:

  • Prepare to Sample. Make a diagram and list of plants to sample and their IDs. Select a plant to sample. Label vial and reaction tube with permanent marker. Flame scissors and end of forceps for 2-3 seconds. Allow to cool for 10 seconds.
  • Sample Leaf. Holding the forceps, cut leaf section approximately 1/4 inch to 1/2 inch long. Place the leaf sample into the appropriate sample vial and secure the cap.
  • Repeat Sampling Process. Repeat the sampling process until all samples have been collected. If you touched the leaves with your gloves, change your gloves before sampling the next plant. 4) Extract. Shake samples vigorously, by hand for ~1 minute. Extraction buffer will likely turn a light green color during this process.
  • Begin the Reaction. Warm heater to 65 °C. Using a small dropper, add a volume of sample extract from the collection vial into the bottom of a reaction tube avoiding any leaf bits. Cap the tube.
  • Dissolve and Mix. Let reaction sit for 30 seconds to allow reagents to dissolve then mix by inverting 2-3 times.
  • Collect Reaction at Bottom of Tube. Collect the reaction mixture into the bottom of the tube by snapping your wrist downward.
  • Place tube in pre-warmed 65°C heat block and heat for 30 minutes. Remove reaction from heat block, place in tube rack, and proceed to results visualization.
  • Dilute and Mix. Using the large dropper, add one volume of dilution buffer to each reaction tube and replace the cap. Mix by lightly flicking the bottom of the tube.
  • Collect at bottom of tube. Collect the reaction mixture into the bottom of the tube by snapping your wrist downward.
  • Insert LFA test strip. Open cap and place in rack. Insert test strip grasping the blue part of the strip and inserting it into the bottom of the 1.5 mL tube. Top third will be sticking out of the tube.
  • Compare to chart. After 10 minutes, observe the results and compare to the chart.

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